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UnitedPlugins FireMaster 1.0.0
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United Plugins & Fire Sonic FireMaster v1.0 MacOSX 3.2.1. JUTorrent. United Plugins & Fire Sonic Fire Master. Скачать. 1.0.0; трек. На хостингеThe present invention relates to the recombinant cloning and expression of the human endothelial cell growth factor-B (ECGF-B) gene. The invention also relates to the use of the human endothelial cell growth factor-B (ECGF-B) gene or gene product in the diagnosis and treatment of vascular diseases.
The human endothelial cell growth factor-B (ECGF-B) was identified as a soluble molecule produced by human melanoma cell line, A375 [Anderson et al. Proc. Natl. Acad. Sci. USA 93:1154-1159 (1996)], that stimulates the growth of cultured endothelial cells [Katayama et al. Biochimica et Biophysica Acta 1364:1-7 (1998) and Harada et al. Biol. Chem. 379:365-370 (1998)]. In these studies, the soluble molecule was purified from culture media using a series of chromatography steps and the amino acid sequence was determined.
The human ECGF-B protein of about 42.5 kD has an NH2-terminal signal peptide, a highly conserved pro-peptide, and an EGF-like domain. The pro-peptide is cleaved from the EGF-like domain during secretion of ECGF-B.
Northern analysis revealed that the human ECGF-B gene was expressed only in human tumor cell lines including melanoma, lung, colon, and sarcoma cells
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