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Traditional microbial identification is performed either by obtaining a pure sample for microscopic observation or using molecular techniques that require several hours of cultivation. To improve diagnosis, we compared two techniques for the detection of biofilm cells. The biofilm cell detection was carried out with real-time quantitative PCR (qRT-PCR) to measure bacterial growth using direct sampling of blood cultures and with digital PCR (dPCR) to detect bacteria in the blood sample after enrichment. For the tests of the blood culture enrichment, 13 bacteremias were obtained from patients with suspected bacteremia. For the tests of direct sampling, 19 samples from positive blood cultures were obtained. To compare the detection methods, we evaluated the time required for detection and the correlation between results using paired samples from the same patient. The time required for direct detection of biofilm cells using qRT-PCR was 26.8 hours. The detection of planktonic cells in blood by dPCR was performed in a time of 12.7 hours. There was a correlation of 1.09 between qRT-PCR and dPCR results, which was not observed in any
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